Hinnen, A., Hicks, J. B., Fink, G. R. (1978) Transformation of yeast. Proceedings of the National Academy of Sciences of the United States of America, 75 (4). pp. 1929-1933. ISSN 00278424 (ISSN)
Abstract
A stable leu2- yeast strain has been transformed to LEU2+ by using a chimeric ColE1 plasmid carrying the yeast leu2 gene. The authors have used recently developed hybridization and restriction endonuclease mapping techniques to demonstrate directly the presence of the transforming DNA in the yeast genome and also to determine the arrangement of the sequences that were introduced. These studies show that ColE1 DNA together with the yeast sequences can integrate into the yeast chromosomes. This integration may be additive or substitutive. The bacterial plasmid sequences, once integrated, behave as a simple Mendelian element. In addition the authors have determined the genetic linkage relationships for each newly introduced LEU2+ allele with the original leu2- allele. These studies show that the transforming sequences integrate not only in the leu2 region but also in several other chromosomal locations.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | colicin e1 colicin factor dna animal experiment fungus genetic transcription in vitro study plasmid yeast Alcohol Oxidoreductases Chromosomes DNA, Bacterial DNA, Recombinant Escherichia coli Genes, Structural Linkage (Genetics) Malates Plasmids Saccharomyces cerevisiae Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Transformation, Genetic |
| Subjects: | organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Hicks lab |
| Depositing User: | Matt Covey |
| Date: | 1978 |
| Date Deposited: | 09 Aug 2013 20:53 |
| Last Modified: | 12 Aug 2013 15:26 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28531 |
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