McEachern, M. J., Hicks, J. B. (1993) Unusually large telomeric repeats in the yeast Candida albicans. Molecular and Cellular Biology, 13 (1). pp. 551-560. ISSN 02707306 (ISSN)
Abstract
We have identified sequences at the telomeres of the yeast Candida albicans and have found that they are composed of tandem copies of a 23-bp sequence. Through the cloning of native telomeric ends and the characterization and cloning of a 'healed' end, we demonstrate that these repeated sequences are sufficient to function as a telomere. All copies of the 23-bp repeat that have been sequenced from a number of C. albicans strains are identical. In contrast, adjacent subtelomeric sequences are variable both between strains and within the WO-1 strain. In the WO-1 strain, the lengths of the telomeres are dependent upon growth temperature and are substantially longer at higher temperatures. Telomere growth is accompanied by increases in the number of the 23-bp repeats present on the telomeric fragments. These results suggest that either telomerase-maintained telomeres can be more complex in structure than was previously imagined or that Candida telomeres are maintained via a telomerase-independent mechanism.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | repetitive dna article candida albicans fungus growth high temperature molecular cloning nonhuman priority journal telomere Base Sequence Chromosomes, Fungal Cloning, Molecular DNA, Fungal Molecular Sequence Data Repetitive Sequences, Nucleic Acid Restriction Mapping Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Temperature |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Hicks lab |
| Depositing User: | Matt Covey |
| Date: | 1993 |
| Date Deposited: | 09 Aug 2013 20:54 |
| Last Modified: | 12 Aug 2013 15:07 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28522 |
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