Rine, J., Strathern, J. N., Hicks, J. B., Herskowitz, I. (1979) A suppressor of mating-type locus mutations in Saccharomyces cerevisiae: Evidence for and identification of cryptic mating-type loci. Genetics, 93 (4). pp. 877-901. ISSN 00166731 (ISSN)
Abstract
A mutation has been identified that suppresses the mating and sporulation defects of all mutations in the mating-type loci of S. cerevisiae. This suppressor, sir1-1, restores mating ability to mat alpha 1 and mat alpha 2 mutants and restores sporulation ability to mat alpha 2 and mata1 mutants. MATa sir1-1 strains exhibit a polar budding pattern and have reduced sensitivity to alpha-factor, both properties of a/alpha diploids. Furthermore, sir1-1 allows MATa/MATa, mat alpha 1/mat alpha/, and MAT alpha/MAT alpha strains to sporulate efficiently. All actions of sir1-1 are recessive to SIR1. The ability of sir1-1 to supply all functions necessary for mating and sporulation and its effects in a cells are explained by proposing that sir1-1 allows expression of mating type loci which are ordinarily not expressed. The ability of sir1-1 to suppress the mat alpha 1-5 mutation is dependent on the HMa gene, previously identified as required for switching of mating types from a to alpha. Thus, as predicted by the cassette model, HMa is functionally equivalent to MAT alpha since it supplies functions of MAT alpha. We propose that sir1-1 is defective in a function. Sir ("Silent-information regulator"), whose role may be to regulate expression of HMa and HM alpha.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | animal experiment fungus gene locus gene repression heredity in vitro study mating saccharomyces cerevisiae Chromosome Mapping Mutation Phenotype Spores, Fungal Support, U.S. Gov't, P.H.S. Suppression, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Hicks lab |
| Depositing User: | Matt Covey |
| Date: | 1979 |
| Date Deposited: | 09 Aug 2013 20:55 |
| Last Modified: | 12 Aug 2013 15:04 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28496 |
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