Hicks, J. B., Hinnen, A., Fink, G. R. (1979) Properties of yeast transformation. Cold Spring Harbor Symposia on Quantitative Biology, 43 (2). pp. 1305-1313. ISSN 00917451 (ISSN)
Abstract
Previous studies showed that the plasmid pYeleu10 could be stably integrated into yeast chromosomes at several locations and, once integrated, the bacterial plasmid sequences (Co1E1) behaved as simple Mendelian elements. In this paper, the authors describe several additional aspects of yeast transformation along with results showing that integrated bacterial plasmid sequences can be retrieved from the yeast genome and reintroduced into bacteria as replicant plasmids; cotransformation of a single cell by two independent plasmids occurs at high frequencies; sequence homology directs the integration of transforming DNA molecules; transformation frequency can be increased dramatically either by converting plasmids from circular plasmids to linear molecules with restriction endonucleases or by creating new recombinant plasmids containing fragments of the indigenous yeast plasmid (ScpI) inserted into the original hybrid plasmids.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | abstract report animal experiment fungus heredity in vitro study transformation yeast Base Sequence Cell Fusion DNA Replication DNA, Circular Escherichia coli Plasmids Recombination, Genetic Saccharomyces cerevisiae Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Transformation, Genetic |
| Subjects: | organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Hicks lab |
| Depositing User: | Matt Covey |
| Date: | 1979 |
| Date Deposited: | 09 Aug 2013 20:57 |
| Last Modified: | 12 Aug 2013 13:56 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28491 |
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