Osten, P., Khatri, L., Perez, J. L., Köhr, G., Giese, G., Daly, C., Schulz, T. W., Wensky, A., Lee, L. M., Ziff, E. B.
(2000)
Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor.
Neuron, 27 (2).
pp. 313-325.
ISSN 08966273 (ISSN)
Abstract
We studied the role of PDZ proteins GRIP, ABP, and PICK1 in GluR2 AMPA receptor trafficking. An epitope-tagged MycGluR2 subunit, when expressed in hippocampal cultured neurons, was specifically targeted to the synaptic surface. With the mutant MycGluR2Δ1-10, which lacks the PDZ binding site, the overall dendritic intracellular transport and the synaptic surface targeting were not affected. However, over time, Myc-GluR2Δ1-10 accumulated at synapses significantly less than MycGluR2. Notably, a single residue substitution, S880A, which blocks binding to ABP/GRIP but not to PICK1, reduced synaptic accumulation to the same extent as the PDZ site truncation. We conclude that the association of GluR2 with ABP and/or GRIP but not PICK1 is essential for maintaining the synaptic surface accumulation of the receptor, possibly by limiting its endocytotic rate.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
AMPA receptor
article
binding site
endocytosis
hippocampus
human
human cell
intracellular transport
mutagenesis
priority journal
synapse vesicle
synaptic transmission
Adaptor Proteins, Signal Transducing
Animals
Binding Sites
Carrier Proteins
Cells, Cultured
Mutagenesis, Site-Directed
Nerve Tissue Proteins
Neurons
Nuclear Proteins
Protein Structure, Tertiary
Proto-Oncogene Proteins c-myc
Rats
Rats, Sprague-Dawley
Receptors, AMPA
Recombinant Fusion Proteins
Sindbis Virus
Synaptic Membranes |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Osten lab |
| Depositing User: |
Matt Covey
|
| Date: |
2000 |
| Date Deposited: |
05 Apr 2013 01:39 |
| Last Modified: |
05 Apr 2013 01:39 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28097 |
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