Plasticity of repetitive DNA sequences within a bacterial (type IV) secretion system component

Aras, R. A., Fischer, W., Perez-Perez, G. I., Crosatti, M., Ando, T., Haas, R., Blaser, M. J. (November 2003) Plasticity of repetitive DNA sequences within a bacterial (type IV) secretion system component. Journal of Experimental Medicine, 198 (9). pp. 1349-1360. ISSN 0022-1007

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Abstract

DNA rearrangement permits bacteria to regulate gene content and expression. In Helicobacter pylori, cagY, which contains an extraordinary number of direct DNA repeats, encodes a surface-exposed subunit of a (type IV) bacterial secretory system. Examining potential DNA rearrangements involving the cagY repeats indicated that recombination events invariably yield in-frame open reading frames, producing alternatively expressed genes. In individual hosts, H. pylori cell populations include strains that produce CagY proteins that differ in size, due to the predicted in-frame deletions or duplications, and elicit minimal or no host antibody recognition. Using repetitive DNA, H. pylori rearrangements in a host-exposed subunit of a conserved bacterial secretion system may permit a novel form of antigenic evasion.

Item Type: Paper
Uncontrolled Keywords: Helicobacter pylori antigenic variation genome plasticity pathogenicity island type IV secretion system HELICOBACTER-PYLORI STRAINS GASTRIC EPITHELIAL-CELLS CAG PATHOGENICITY ISLAND ANTIGENIC VARIATION ESCHERICHIA-COLI HOST-CELLS IN-VIVO GENETIC-TRANSFORMATION PROTEIN SURFACE
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > bacteria
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: November 2003
Date Deposited: 02 Apr 2013 15:30
Last Modified: 02 Apr 2013 15:30
PMCID: PMC2194252
Related URLs:
URI: https://repository.cshl.edu/id/eprint/27998

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