Ono, T., Losada, A., Hirano, M., Myers, M. P., Neuwald, A. F., Hirano, T.
(October 2003)
Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
Cell, 115 (1).
pp. 109-121.
ISSN 0092-8674
Abstract
The canonical condensin complex (henceforth condensin I) plays an essential role in mitotic chromosome assembly and segregation from yeast to humans. We report here the identification of a second condensin complex (condensin II) from vertebrate cells. Condensins I and II share the same pair of structural maintenance of chromosomes (SMC) subunits but contain different sets of non-SMC subunits. siRNA-mediated depletion of condensin I- or condensin II-specific subunits in HeLa cells produces a distinct, highly characteristic defect in chromosome morphology. Simultaneous depletion of both complexes causes the severest defect. In Xenopus egg extracts, condensin I function is predominant, but lack of condensin II results in the formation of irregularly shaped chromosomes. Condensins I and II show different distributions along the axis of chromosomes assembled in vivo and in vitro. We propose that the two condensin complexes make distinct mechanistic contributions to mitotic chromosome architecture in vertebrate cells.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
SISTER-CHROMATID COHESION
XENOPUS EGG EXTRACTS
PROTEIN COMPLEXES
13S
CONDENSIN
SMC PROTEINS
SEGREGATION
MEIOSIS
DNA
IDENTIFICATION
MITOSIS |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organs, tissues, organelles, cell types and functions > organelles, types and functions
organs, tissues, organelles, cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hirano lab |
| Depositing User: |
Matt Covey
|
| Date: |
October 2003 |
| Date Deposited: |
02 Apr 2013 13:56 |
| Last Modified: |
02 Apr 2013 13:56 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27991 |
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