Nestin expression in hair follicle sheath progenitor cells

Li, L. N., Mignone, J., Yang, M., Matic, M., Penman, S., Enikolopov, G., Hoffman, R. M. (August 2003) Nestin expression in hair follicle sheath progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 100 (17). pp. 9958-9961. ISSN 0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/12904579

DOI: 10.1073/pnas.1733025100

Abstract

The intermediate filament protein, nestin, marks progenitor cells of the CNS. Such CNS stem cells are selectively labeled by placing GFP under the control of the nestin regulatory sequences. During early anagen or growth phase of the hair follicle, nestin-expressing cells, marked by GFP fluorescence in nestin-GFP transgenic mice, appear in the permanent upper hair follicle immediately below the sebaceous glands in the follicle bulge. This is where stem cells for the hair follicle outer-root sheath are thought to be located. The relatively small, oval-shaped, nestin-expressing cells in the bulge area surround the hair shaft and are interconnected by short dendrites. The precise locations of the nestin-expressing cells in the hair follicle vary with the hair cycle. During telogen or resting phase and in early anagen, the GFP-positive cells are mainly in the bulge area. However, in mid- and late anagen, the GFP-expressing cells are located in the upper outer-root sheath as well as in the bulge area but not in the hair matrix bulb. These observations show that the nestin-expressing cells form the outer-root sheath. Results of the immunohistochemical staining showed that nestin, GFP, keratin 5/8, and keratin 15 colocalize in the hair follicle bulge cells, outer-root sheath cells, and basal cells of the sebaceous glands. These data indicate that nestin-expressing cells, marked by GFP, in the hair follicle bulge are indeed progenitors of the follicle outer-root sheath. The expression of the unique protein, nestin, in both neural stem cells and hair follicle stem cells suggests their possible relation.

Item Type:	Paper
Uncontrolled Keywords:	stem cells hair cycle neurological GFP imaging STEM-CELLS GENE
Subjects:	organs, tissues, organelles, cell types and functions > organs types and functions > brain bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > nestin bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:	Enikolopov, Grigori N. Mignone, John L.
Communities:	CSHL labs > Enikopolov lab
Depositing User:	Matt Covey
Date:	August 2003
Date Deposited:	10 Jun 2013 14:20
Last Modified:	10 Sep 2019 18:38
PMCID:	PMC187900
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27970

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