Debnath, J., Muthuswamy, S. K., Brugge, J. S.
(July 2003)
Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures.
Methods, 30 (3).
pp. 256-268.
ISSN 1046-2023
Abstract
The three-dimensional culture of MCF-10A mammary epithelial cells on a reconstituted basement membrane results in formation of polarized, growth-arrested acini-like spheroids that recapitulate several aspects of glandular architecture in vivo. Oncogenes introduced into MCF-10A cells disrupt this morphogenetic process, and elicit distinct morphological phenotypes. Recent studies analyzing the mechanistic basis for phenotypic heterogeneity observed among different oncogenes (e.g., ErbB2, cyclin D1) have illustrated the utility of this three-dimensional culture system in modeling the biological activities of cancer genes, particularly with regard to their ability to disrupt epithelial architecture during the early aspects of carcinoma formation. Here we provide a collection of protocols to culture MCF-10A cells, to establish stable pools expressing a gene of interest via retroviral infection, as well as to grow and analyze MCF-10A cells in three-dimensional basement membrane culture. (C) 2003 Elsevier Science (USA). All rights reserved.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
three-dimensional culture
MCF-10A
mammary acini
Oncogenes
luminal
apoptosis
glandular architecture
HUMAN-BREAST-CANCER
GENE-EXPRESSION PATTERNS
CELL-LINE
APOPTOSIS
SURVIVAL
POLARITY
MODELS
DIFFERENTIATION
TRANSFORMATION
PROLIFERATION |
| Subjects: |
Investigative techniques and equipment
Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > tissues types and functions > mammary gland
organs, tissues, organelles, cell types and functions > tissues types and functions |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Muthuswamy lab |
| Depositing User: |
Matt Covey
|
| Date: |
July 2003 |
| Date Deposited: |
11 Jun 2013 20:41 |
| Last Modified: |
11 Jun 2013 20:41 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27939 |
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