Lipovac, M. N., Holland, T., Poleksic, A., Killian, C., Lajtha, A. (May 2003) The possible role of glutamate uptake in metaphit-induced seizures. Neurochemical Research, 28 (5). pp. 723-731. ISSN 0364-3190
Abstract
In a study of the possible mechanism of action of metaphit and phencyclidine in the brain, the uptake of glutamate at the luminal side of the blood-brain barrier (BBB) was studied by means of an in situ brain perfusion technique in normal guinea pigs and in those pretreated with metaphit. Metaphit, an isothiocyanate analog of phencyclidine (PCP), induces time-dependent epileptogenic changes in the electroencephalogram in guinea pig, reaching a maximum 18-24 h after metaphit administration (50 mg/kg IP). In metaphit-pretreated animals a significant reduction of glutamate BBB uptake was found, in comparison with that of controls. Reduction of glutamate transport from blood to brain ranged from 77% to 79% in all brain structures studied. This inhibition was probably due to changes in the properties of saturable components responsible for transport of glutamate across the BBB. Kinetic measurements revealed a saturable amino acid influx into the parietal cortex, caudate nucleus, and hippocampus, with a Km between 3.1 and 5.1 muM, and the V-max ranging from 14.3 to 27.8 pmol(-1) g(-1). The nonsaturable component, K-id, was statistically different from zero, ranging from 1.47 to 2.00 muM min(-1) g(-1). Influx of glutamate into the brain was not altered in the presence of 1 mM D-aspartate, but it was significantly inhibited in the presence of 1 mM L-aspartate. We conclude that the cerebrovascular permeability of circulating glutamate is due to the presence of a higher-capacity saturable receptor and/or a carrier-mediated transport system (75%) and also a low-capacity diffusion transport system (25%) for the glutamate located at the luminal side of the BBB. The glutamate transport system is probably fully saturated at physiological plasma glutamate concentrations.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | seizures blood brain barrier glutamate transport BLOOD-BRAIN-BARRIER GUINEA-PIG BRAIN ALPHA-AMINOISOBUTYRIC-ACID AMINO-ACIDS AUDIOGENIC-SEIZURES PHENCYCLIDINE RECEPTORS KINETIC-ANALYSIS TRANSPORT MICE CAPILLARY |
| Subjects: | diseases & disorders > nervous system diseases and disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification diseases & disorders > nervous system diseases and disorders > epilepsy bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor |
| CSHL Authors: | |
| Communities: | CSHL labs > Neuwald lab |
| Depositing User: | Matt Covey |
| Date: | May 2003 |
| Date Deposited: | 26 Jun 2013 19:36 |
| Last Modified: | 26 Jun 2013 19:36 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27918 |
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