Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species

Fouts, D. E., Mongodin, E. F., Mandrell, R. E., Miller, W. G., Rasko, D. A., Ravel, J., Brinkac, L. M., Deboy, R. T., Parker, C. T., Daugherty, S. C., Dodson, R. J., Scott Durkin, A., Madupu, R., Sullivan, S. A., Shetty, J. U., Ayodeji, M. A., Shvartsbeyn, A., Schatz, M. C., Badger, J. H., Fraser, C. M., Nelson, K. E. (2005) Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species. PLoS Biology, 3 (1). ISSN 15449173 (ISSN)

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DOI: 10.1371/journal.pbio.0030015


Sequencing and comparative genome analysis of four strains of Campylobacter including C. lari RM2100, C. upsaliensis RM3195, and C. coli RM2228 has revealed major structural differences that are associated with the insertion of phage- and plasmid-like genomic islands, as well as major variations in the lipooligosaccharide complex. Poly G tracts are longer, are greater in number, and show greater variability in C. upsaliensis than in the other species. Many genes involved in host colonization, including racR/S, cadF, cdt, ciaB, and flagellin genes, are conserved across the species, but variations that appear to be species specific are evident for a lipooligosaccharide locus, a capsular (extracellular) polysaccharide locus, and a novel Campylobacter putative licABCD virulence locus. The strains also vary in their metabolic profiles, as well as their resistance profiles to a range of antibiotics. It is evident that the newly identified hypothetical and conserved hypothetical proteins, as well as uncharacterized two-component regulatory systems and membrane proteins, may hold additional significant information on the major differences in virulence among the species, as well as the specificity of the strains for particular hosts. Copyright: © 2005 Fouts et al.

Item Type: Paper
Uncontrolled Keywords: lipooligosaccharide membrane protein polysaccharide bacterial protein article bacterial gene bacterial genetics bacterial genome bacterial virulence Campylobacter coli Campylobacter lari Campylobacter upsaliensis controlled study gene insertion gene sequence gene structure genetic conservation genetic variability genome analysis host nonhuman structure analysis animal bird bird disease Campylobacter cattle cattle disease classification comparative study genetics microbiology molecular genetics pathogenicity phylogeny sequence alignment sequence homology statistical model swine swine disease virulence Charadrii Animals Bacterial Proteins Bird Diseases Birds Cattle Diseases Genome, Bacterial Likelihood Functions Molecular Sequence Data Sequence Homology, Amino Acid Swine Diseases
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > bacteria
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
CSHL Authors:
Communities: CSHL labs > Schatz lab
Depositing User: Matt Covey
Date: 2005
Date Deposited: 15 Mar 2013 17:52
Last Modified: 15 Mar 2013 17:52
PMCID: PMC539331
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