Construction of restriction enzyme fragment libraries containing DNA from human adenovirus types 2 and 5

Stenlund, A., Perricaudet, M., Tiollais, P., Pettersson, U. (June 1980) Construction of restriction enzyme fragment libraries containing DNA from human adenovirus types 2 and 5. Gene, 10 (1). pp. 47-52. ISSN 0378-1119 (Print)0378-1119 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6250946

DOI: 10.1016/0378-1119(80)90142-0

Abstract

Restriction-fragment libraries containing adenovirus type 2 (Ad2) DNA have been constructed, using the pBR322 plasmid (Bolivar et al., 1977) as a vector. Clones have been isolated which contain all the HindIII fragments of Ad2 DNA except the terminal G- and K-fragments inserted into the HindIII cleavage site of the vector. All the 13 SmaI-fragments of Ad2 DNA were separately inserted into the PstI site of the pBR322 vector after addition of homopolymeric poly(dG) tails to the fragments and poly(dC) tails to the linearized plasmid. Two large fragments of adenovirus type 5 (AD5) DNA, located between map positions 17.0 and 59.5 and between map positions 59.5 and 97.3, respectively, were cloned using bacteriophage lambda as a vector. All clones, which are described in the present report, are available upon request.

Item Type:	Paper
Uncontrolled Keywords:	Adenoviruses, Human/ genetics Bacteriophage lambda/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Escherichia coli/genetics Genetic Vectors Plasmids
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organism description > virus > adeno associated virus organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme organism description > virus
CSHL Authors:	Stenlund, Arne
Communities:	CSHL labs > Stenlund lab
Depositing User:	Matt Covey
Date:	June 1980
Date Deposited:	12 Mar 2013 18:54
Last Modified:	12 Mar 2013 18:54
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27773

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