Getting around lethality with inducible Cre-mediated excision

Garcia, E. L., Mills, A. A. (April 2002) Getting around lethality with inducible Cre-mediated excision. Seminars in Cell & Developmental Biology, 13 (2). pp. 151-158. ISSN 1084-9521

DOI: 10.1016/S1084-9521(02)00019-8


For almost two decades, geneticists have manipulated the mouse genome to explore gene function and to understand the role of individual genes in normal development and the disease process. Manipulating the mouse genome in a precise manner begins with a pluripotent embryonic stem (ES) cell isolated from the inner cell mass of a developing blastocyst.1. and 2. The genome of the ES cell can be modified by gene targeting—a procedure involving homologous recombination between the endogenous target gene and a modified version of that gene present in the introduced targeting construct; accurate recombination at the target locus replaces the endogenous gene with the modified version. The targeting vector is extremely versatile; it can be used to ablate or ‘knock-out’ gene function by removing an essential region of the target gene, to introduce subtle mutations in the target gene, to insert or ‘knock-in’ an entirely different gene at the target locus, and to generate megabase chromosome rearrangements within defined regions of the genome. These approaches provide researchers with tools that can be used to address a diverse array of biological questions.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Mills lab
Depositing User: Matt Covey
Date: April 2002
Date Deposited: 11 Mar 2013 20:00
Last Modified: 11 Mar 2013 20:00
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