Lee, R. C., Hammell, C. M., Ambros, V. (2006) Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans. RNA, 12 (4). pp. 589-597. ISSN 13558382 (ISSN)
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Abstract
C. elegans contains numerous small RNAs of ∼21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2, RDE-3, RDE-4, RRF-1, and RRF-3. The tiny noncoding (tncRNAs) are similar to endo-siRNAs in their biogenesis except that they are derived from noncoding sequences. These endo-siRNA- and tncRNA-based endogenous RNAi pathways involve some components, including DCR-1 and RDE-4, that are shared with exogenous RNAi, and some components, including RRF-3 and ERI-1, that are specific to endogenous RNAi. rrf-3 and eri-1 mutants are enhanced for some silencing processes and defective for others, suggesting cross-regulatory interactions between RNAi pathways in C. elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes. Copyright © 2006 RNA Society.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Caenorhabditis elegans microRNA Noncoding RNA RdRp RNAi siRNA small interfering RNA amino acid sequence animal experiment article biogenesis controlled study gene interaction gene mutation gene silencing genetic transcription microarray analysis nonhuman priority journal RNA interference Animals Base Sequence Blotting, Northern Mutation Oligonucleotide Probes Animalia |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi organism description > animal |
| CSHL Authors: | |
| Communities: | CSHL labs > Hammell C. lab |
| Depositing User: | Matt Covey |
| Date: | 2006 |
| Date Deposited: | 11 Mar 2013 16:56 |
| Last Modified: | 09 Nov 2017 21:26 |
| PMCID: | PMC1421084 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27741 |
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