Dutta, A., Din, S., Brill, S. J., Stillman, B.
(1991)
Phosphorylation of replication protein A: A role for cdc2 kinase in G1/S regulation.
Cold Spring Harbor Symposia on Quantitative Biology, 56.
pp. 315-324.
ISSN 00917451 (ISSN)
Abstract
Among the various phases of the cell cycle, we have concentrated on the regulation of the S phase. Our approach is to define biochemically the components of the DNA replication apparatus and then to analyze how the various components are regulated as cells enter, execute, and exit the S phase. Working from the substrate, namely the DNA replication apparatus, backward to the regulatory molecules would complement the more common genetic approach, which first uncovers the regulatory molecules and then works forward to the regulated substrates.
Fusion of animal cells at various stages in the cell cycle has shown that S-phase nuclei contain a positive activator of DNA replication which is absent in G1 cells and which could hasten the onset of S phase in G1 nuclei (Rao and Johnson 1970). Hence, one of our goals is to identify biochemically this positive activator, tentatively designated S-phase promoting factor (SPF). Furthermore, once...
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
cyclin b
phosphoamino acid
phosphoprotein
protein kinase
cell cycle g1 phase
cell cycle s phase
cell division
conference paper
enzyme purification
hela cell
human
human cell
immunoblotting
kidney cell
nonhuman
priority journal
protein phosphorylation
saccharomyces cerevisiae
Cell Cycle
Cyclins
DNA Replication
DNA-Binding Proteins
Fungal Proteins
Interphase
Models, Biological
Phosphorylation
Protein p34cdc2
S Phase
Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S. |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Stillman lab |
| Highlight: |
Stillman, Bruce W. |
| Depositing User: |
Matt Covey
|
| Date: |
1991 |
| Date Deposited: |
08 Mar 2013 18:34 |
| Last Modified: |
20 Jun 2017 20:13 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27736 |
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