DNA replication and the cell cycle

Stillman, B., Bell, S. P., Dutta, A., Marahrens, Y. (1992) DNA replication and the cell cycle. Regulation of the eukaryotic cell cycle. Ciba Foundation symposium, 170. 147-156; discussion 156. ISSN 03005208 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1336449
DOI: 10.1002/9780470514320


The replication of DNA in the eukaryotic cell cycle is one of the most highly regulated events in cell growth and division. Biochemical studies on the replication of the genome of the small DNA virus simian virus 40 (SV40) have resulted in the identification of a number of DNA replication proteins from human cells. One of these, Replication Protein A (RPA), was phosphorylated in a cell cycle-dependent manner, beginning at the onset of DNA replication. RPA was phosphorylated in vitro by the cell cycle-regulated cdc2 protein kinase. This kinase also stimulated the unwinding of the SV40 origin of DNA replication during initiation of DNA replication in vitro, suggesting a mechanism by which cdc2 kinase may regulate DNA replication. Functional homologues of the DNA replication factors have been identified in extracts from the yeast Saccharomyces cerevisiae, enabling a genetic characterization of the role of these proteins in the replication of cellular DNA. A cellular origin binding protein had not been characterized. To identify proteins that function like T antigen at cellular origins of DNA replication, we examined the structure of a yeast origin of DNA replication in detail. This origin consists of four separate functional elements, one of which is essential. A multiprotein complex that binds to the essential element has been identified and purified. This protein complex binds to all known cellular origins from S. cerevisiae and may function as an origin recognition complex.

Item Type: Paper
Uncontrolled Keywords: animal cell cycle DNA replication genetics human physiology review Simian virus 40 virus replication Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: Matt Covey
Date: 1992
Date Deposited: 08 Mar 2013 18:37
Last Modified: 20 Jun 2017 13:28
Related URLs:
URI: https://repository.cshl.edu/id/eprint/27727

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