The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo

Bestman, J. E., Cline, H. T. (December 2008) The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A, 105 (51). 20494-20499 .

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Abstract

Visual system development requires experience-dependent mechanisms that regulate neuronal structure and function, including dendritic arbor growth, synapse formation, and stabilization. Although RNA binding proteins have been shown to affect some forms of synaptic plasticity in adult animals, their role in the development of neuronal structure and functional circuitry is not clear. Using two-photon time-lapse in vivo imaging and electrophysiology combined with morpholino-mediated knockdown and expression of functional deletion mutants, we demonstrate that the mRNA binding protein, cytoplasmic polyadenylation element binding protein1 (CPEB1), affects experience-dependent neuronal development and circuit formation in the visual system of . These data indicate that sensory experience controls circuit development by regulating translational activity of mRNAs.

Item Type:	Paper
Uncontrolled Keywords:	circuit integration experience-dependent plasticity In vivo imaging visual system whole-cell recording
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > animal > Frog bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics organs, tissues, organelles, cell types and functions organs, tissues, organelles, cell types and functions > tissues types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex organism description > animal > Frog > xenopus
CSHL Authors:	Bestman, Jennifer E. Cline, Hollis T.
Communities:	CSHL labs > Cline lab
Depositing User:	Matt Covey
Date:	23 December 2008
Date Deposited:	25 Feb 2013 16:19
Last Modified:	08 Nov 2017 20:57
PMCID:	PMC2629308
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27612

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