Neuronal nitric oxide synthase contributes to the regulation of hematopoiesis

Krasnov, P., Michurina, T. V., Packer, M. A., Stasiv, Y., Nakaya, N., Moore, K. A., Drazan, K. E., Enikolopov, G. N. (March 2008) Neuronal nitric oxide synthase contributes to the regulation of hematopoiesis. Molecular Medicine, 14 (3-4). pp. 141-149. ISSN 1076-1551

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URL: http://www.ncbi.nlm.nih.gov/pubmed/18091979

DOI: 10.2119/2007-00011.Krasnov

Abstract

Nitric oxide (NO) signaling is important for the regulation of hematopoiesis. However, the role of individual NO synthase (NOS) isoforms is unclear. Our results indicate that the neuronal NOS isoform (nNOS) regulates hematopolesis in vitro and in vivo. nNOS is expressed in adult bone marrow and fetal liver and is enriched in stromal cells. There is a strong correlation between expression of nNOS in a panel of stromal cell lines established from bone marrow and fetal liver and the ability of these cell lines to support hematopoietic stem cells; furthermore, NO donor can further increase this ability. The number of colonies generated in vitro from the bone marrow and spleen of nNOS-null mutants is increased relative to wild-type or inducible- or endothelial NOS knockout mice. These results describe a new role for nNOS beyond its action in the brain and muscle and suggest a model where nNOS, expressed in stromal cells, produces NO which acts as a paracrine regulator of hematopoietic stem cells.

Item Type:	Paper
Uncontrolled Keywords:	STEM-CELL ACTIVITY BONE-MARROW-CELLS MESSENGER-RNA DROSOPHILA DEVELOPMENT ADULT NEUROGENESIS BIOLOGICAL SAMPLES PROGENITOR CELLS GENE-EXPRESSION STROMAL CELLS PC12 CELLS
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > nitric oxide synthase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:	Enikolopov, Grigori N. Krasnov, Peter Michurina, Tatyana V. Nakaya, Naoki Packer, Michael A. Stasiv, Yuri
Communities:	CSHL labs > Enikopolov lab
Depositing User:	Matt Covey
Date:	March 2008
Date Deposited:	26 Feb 2013 15:00
Last Modified:	26 Feb 2013 15:00
PMCID:	PMC2140250
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27583

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