Accurate proteome-wide protein quantification from high-resolution N-15 mass spectra

Khan, Z., Amini, S., Bloom, J. S., Ruse, C., Caudy, A. A., Kruglyak, L., Singh, M., Perlman, D. H., Tavazoie, S. (2011) Accurate proteome-wide protein quantification from high-resolution N-15 mass spectra. Genome Biology, 12 (12). ISSN 1474-7596

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DOI: 10.1186/gb-2011-12-12-r122


In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of N-15-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing N-15 spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.

Item Type: Paper
Uncontrolled Keywords: escherichia-coli quantitative proteomics stationary-phase gene-expression spectrometry identification conversion algorithm culture rates
Subjects: bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
biotechnology > chromatography > protein purification
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: 2011
Date Deposited: 06 Feb 2013 14:53
Last Modified: 06 Feb 2013 14:53
PMCID: PMC3334617
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