Parla, J., Kramer, M. R., McCombie, W. R. (2011) High-Throughput Sequencing. In: Microbial Forensics (Second Edition). Academic Press, San Diego, pp. 461-478.
Abstract
Summary Genomic sequence data have been a major driver of progress in microbiology in the past three decades, since Frederick Sanger published his chain termination DNA sequencing method in 1977 and the genome sequence of bacteriophage [lambda] in 1982, which was the first detailed whole genome sequencing project. The wealth of information provided by DNA sequencing technology and the bioinformatic resources developed to manage and analyze sequence data have enabled biological studies greatly. This chapter discusses the particular impact of sequencing advances on microbiology. A discussion of advanced DNA sequencing technologies distinct from the Sanger dideoxy chain termination method, which are also unparalleled in scalability and throughput, is also presented. These next-generation sequencing techniques have promoted creative and important biological studies that were not feasible, efficient, or comprehensive with Sanger chemistry or alternative techniques, such as metagenomics, chromatin immunoprecipitation studies, and new pathogen identification.
| Item Type: | Book Section |
|---|---|
| Subjects: | Investigative techniques and equipment Investigative techniques and equipment > assays Investigative techniques and equipment > whole exome sequencing Investigative techniques and equipment > assays > whole exome sequencing |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab CSHL Post Doctoral Fellows |
| Depositing User: | Matt Covey |
| Date: | 2011 |
| Date Deposited: | 05 Feb 2013 22:03 |
| Last Modified: | 03 May 2013 13:29 |
| URI: | https://repository.cshl.edu/id/eprint/27189 |
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