Serial two-photon tomography for automated ex vivo mouse brain imaging

Ragan, T., Kadiri, L. R., Venkataraju, K. U., Bahlmann, K., Sutin, J., Taranda, J., Arganda-Carreras, I., Kim, Y., Seung, H. S., Osten, P. (March 2012) Serial two-photon tomography for automated ex vivo mouse brain imaging. Nature Methods, 9 (3). pp. 255-258. ISSN 15487091 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/22245809

DOI: 10.1038/nmeth.1854

Abstract

Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

Item Type:	Paper
Subjects:	Investigative techniques and equipment organism description > animal organs, tissues, organelles, cell types and functions > organs types and functions > brain Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > imaging organism description > animal > mammal Investigative techniques and equipment > microscopy organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent Investigative techniques and equipment > microscopy > flourescence microscopy > two-photon excitation microscopy
CSHL Authors:	Kadiri, Lolahon R. Kim, Yongsoo Osten, Pavel Taranda, Julian Umadevi Venkataraju, Kannan
Communities:	CSHL Post Doctoral Fellows CSHL labs > Osten lab Stanley Institute for Cognitive Genomics
Highlight:	Osten, Pavel
Depositing User:	Matt Covey
Date:	March 2012
Date Deposited:	29 Jan 2013 17:29
Last Modified:	03 Oct 2019 14:48
PMCID:	PMC3297424
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27025

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