Growth suppression of esophageal cancer cells by p16 INK4 and p15 INK4B in vitro

Zhou, X., Suzuki, H., Lei, J., Cole, K., Yin, J., Suzuki, Y., Abraham, J. M., Shimada, Y., Imamura, M., Chan, T., Hannon, G. J., Meltzer, S. J. (1996) Growth suppression of esophageal cancer cells by p16 INK4 and p15 INK4B in vitro. Cancer Journal from Scientific American, 2 (4). pp. 221-224. ISSN 10814442 (ISSN)



PURPOSE: We investigate whether p16 INK4 and p15 INK4B inhibit cellular proliferation and exert a growth suppressive effect on esophageal cancer cells. MATERIALS AND METHODS: The growth suppressive effects of p6 INK4 and p15 INK4B were evaluated by transfecting vectors containing the p16 INK4 cDNA or the p15 INK4B cDNA, or both, constitutively driven by a cytomegalovirus promoter, into two human esophageal cancer cell lines containing or lacking endogenous p16 INK4 and/or p15 INK4B. RESULTS: These experiments demonstrated that in both cells lines tested, the numbers of cells surviving dramatically decreased in p16 INK4-transfected and p15 INK4B-transfected cells compared with control vector-transfected cells. There was no significant difference in the degree of growth inhibition between p16 INK4-transfected and p15 INK4B-transfected cell lines. CONCLUSIONS: These results suggest that p16 INK4 and p15 INK4B play important roles in the initiation or promotion of esophageal cancer. The inactivation of p16 INK4 and p15 INK4B may contribute to uncontrolled growth in human cancer.

Item Type: Paper
Uncontrolled Keywords: CDKI Esophageal carcinoma Growth suppression MTS1 MTS2 p15 INK4B p16 INK4 cyclin dependent kinase inhibitor 2B protein p16INK4a article cell culture cell proliferation esophagus tumor genetic transfection genetics human in vitro study metabolism pathology physiology squamous cell carcinoma Carcinoma, Squamous Cell Cyclin-Dependent Kinase Inhibitor p15 Cyclin-Dependent Kinase Inhibitor p16 Esophageal Neoplasms Humans Transfection Tumor Cells, Cultured
Subjects: diseases & disorders > cancer
diseases & disorders
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1996
Date Deposited: 09 Jan 2013 17:32
Last Modified: 09 Jan 2013 17:32

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