Drop-delay measurement using enzyme-coated particles

Lazebnik, Y. A., Poletaev, A. I., Zenin, V. V. (1992) Drop-delay measurement using enzyme-coated particles. Cytometry, 13 (145159). pp. 649-652. ISSN 0196-4763

URL: http://www.ncbi.nlm.nih.gov/pubmed/1451596


A simple technique is suggested for the measurement of drop delay for flow sorting. While the flow cytometer was set to sort a fixed number of particles, the drop-delay setting was changed step by step, and at each step the HRP-coupled particles were sorted into a well of an immunoassay strip. Then the HRP activity of the sorted samples was revealed by routine methods. The maximum level of the enzyme activity shows the proper drop-delay setting. Determination of the drop-delay setting takes only a few minutes. The technique is independent of the type of flow cytometer and does not require any additional equipment.

Item Type: Paper
Uncontrolled Keywords: Animals Benzimidazoles Blood Cell Count Cell Separation Flow Cytometry Fluorescein-5-isothiocyanate Horseradish Peroxidase Microscopy, Fluorescence Nephelometry and Turbidimetry Ranidae Time Factors
Subjects: Investigative techniques and equipment
CSHL Authors:
Communities: CSHL labs > Labeznik lab
Depositing User: Matt Covey
Date: 1992
Date Deposited: 12 Dec 2012 17:32
Last Modified: 12 Dec 2012 17:32
URI: https://repository.cshl.edu/id/eprint/26406

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