Lazebnik, Y. A., Poletaev, A. I., Zenin, V. V. (1992) Drop-delay measurement using enzyme-coated particles. Cytometry, 13 (145159). pp. 649-652. ISSN 0196-4763
Abstract
A simple technique is suggested for the measurement of drop delay for flow sorting. While the flow cytometer was set to sort a fixed number of particles, the drop-delay setting was changed step by step, and at each step the HRP-coupled particles were sorted into a well of an immunoassay strip. Then the HRP activity of the sorted samples was revealed by routine methods. The maximum level of the enzyme activity shows the proper drop-delay setting. Determination of the drop-delay setting takes only a few minutes. The technique is independent of the type of flow cytometer and does not require any additional equipment.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Benzimidazoles Blood Cell Count Cell Separation Flow Cytometry Fluorescein-5-isothiocyanate Horseradish Peroxidase Microscopy, Fluorescence Nephelometry and Turbidimetry Ranidae Time Factors |
| Subjects: | Investigative techniques and equipment |
| CSHL Authors: | |
| Communities: | CSHL labs > Labeznik lab |
| Depositing User: | Matt Covey |
| Date: | 1992 |
| Date Deposited: | 12 Dec 2012 17:32 |
| Last Modified: | 12 Dec 2012 17:32 |
| URI: | https://repository.cshl.edu/id/eprint/26406 |
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