Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry

Spector, D. L. (1984) Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry. Biology of the Cell, 51 (1). pp. 109-112. ISSN 0248-4900

DOI: 10.1111/j.1768-322X.1984.tb00289.x


The in situ localization of U1 and U2 snRNPs was examined, using autoantibodies directed against each of these snRNPs, by immunofluorescence and immunoelectron microscopy. This study has shown U1 and U2 snRNPs to colocalize in the nuclei of PtK2 cells. Thirty to fifty immunostained clusters were observed per interphase nucleus. This study suggests these nuclear protein clusters to be the sites of RNA processing.

Item Type: Paper
Uncontrolled Keywords: Animals Antibodies, Monoclonal Autoantibodies Cell Line Fluorescent Antibody Technique Macropodidae RNA RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Subjects: bioinformatics > genomics and proteomics > analysis and processing > DNA RNA Processing
organs, tissues, organelles, cell types and functions > organelles, types and functions > nucleus
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date: 1984
Date Deposited: 10 Dec 2012 20:04
Last Modified: 29 Jan 2015 20:56
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