Detection of secreted peptides by using hypothesis-driven multistage mass spectrometry

Kalkum, Markus, Lyon, Gholson J., Chait, Brian T. (2003) Detection of secreted peptides by using hypothesis-driven multistage mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America, 100 (5). pp. 2795-2800. ISSN 0027-8424

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DOI: 10.1073/pnas.0436605100


A method is presented for the rapid detection and characterization of trace amounts of peptides secreted from microorganisms, including pheromones, virulence factors, and quorum-sensing peptides. The procedure, based on targeted multistage MS, uses a novel matrix-assisted laser desorptionionization-ion trap mass spectrometer to overcome limitations of current MS methods (limited dynamic range, signal suppression effects, and chemical noise) that impair observation of low abundance peptides from complex biological matrixes. Here, secreted peptides that are hypothesized to be present in the supernatant, but that may not be sufficiently abundant to be observed in single-stage mass spectra, are subjected to multistage MS. Highly specific fragmentation signatures enable unambiguous identification of the peptides of interest and differentiation of the signals from the background. As examples, we demonstrate the rapid (<1 min) determination of the mating type of cells in colonies of Saccharomyces cerevisiae and the elucidation of autoinducing peptides (AIPs) from supernatants of pathogenic Staphylococci. We confirm the primary structures of the agrD encoded cyclic AIPs of Staphylococcus aureus for groups I, II, and IV and provide direct evidence that the native group-III AIP is a heptapeptide (INCDFLL). We also show that the homologous peptide from Staphylococcus intermedius is a nonapeptide (RIPTSTGFF) with a lactone ring formed through condensation of the serine side chain with the C terminus of the peptide. This is the first demonstration of cyclization in a staphylococcal AIP that occurs via lactone formation. These examples demonstrate the analytical power of the present procedure for characterizing secreted peptides and its potential utility for identifying microorganisms.

Item Type: Paper
Uncontrolled Keywords: Mass Spectrometry Peptides Signal Transduction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Staphylococcus Staphylococcus aureus Virulence
Subjects: Investigative techniques and equipment > spectroscopy > mass spectrometry
CSHL Authors:
Communities: CSHL labs > Lyon lab
Depositing User: Matt Covey
Date: 2003
Date Deposited: 10 Dec 2012 20:12
Last Modified: 10 Sep 2019 18:42
PMCID: PMC151420

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