Sordella, R., Classon, M., Hu, K. Q., Matheson, S. F., Brouns, M. R., Fine, B., Zhang, L., Takami, H., Yamada, Y., Settleman, J. (May 2002) Modulation of CREB activity by the Rho GTPase regulates cell and organism size during mouse embryonic development. Developmental Cell, 2 (5). pp. 553-65. ISSN 1534-5807
Abstract
Rho GTPases regulate several aspects of tissue morphogenesis during animal development. We found that mice lacking the Rho-inhibitory protein, p190-B RhoGAP, are 30% reduced in size and exhibit developmental defects strikingly similar to those seen in mice lacking the CREB transcription factor. In p190-B RhoGAP-deficient mice, CREB phosphorylation is substantially reduced in embryonic tissues. Embryo-derived cells contain abnormally high levels of active Rho protein, are reduced in size, and exhibit defects in CREB activation upon exposure to insulin or IGF-1. The cell size defect is rescued by expression of constitutively activated CREB, and in wild-type cells, expression of activated Rho or dominant-negative CREB results in reduced cell size. Together, these results suggest that activity of the Rho GTPase modulates a signal from insulin/IGFs to CREB that determines cell size and animal size during embryogenesis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Body Constitution Cell Size Cyclic AMP Response Element-Binding Protein metabolism DNA-Binding Proteins Embryonic and Fetal Development GTPase Activating Proteins Guanine Nucleotide Exchange Factors deficiency genetics metabolism Insulin metabolism Insulin Receptor Substrate Proteins Mice Mice, Knockout Mitogen-Activated Protein Kinases metabolism Models Biological Nuclear Proteins deficiency genetics metabolism Phenotype Phosphoproteins metabolism Phosphorylation Repressor Proteins Signal Transduction rho GTP-Binding Proteins metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTPase organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation organism description > animal > developmental stage organism description > animal > developmental stage > embryo organism description > animal > mammal > rodent > mouse |
| CSHL Authors: | |
| Communities: | CSHL labs > Sordella lab |
| Depositing User: | CSHL Librarian |
| Date: | May 2002 |
| Date Deposited: | 09 May 2012 17:51 |
| Last Modified: | 13 Mar 2013 16:19 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26244 |
Actions (login required)
 |
Administrator's edit/view item |