Serotonin-sensitive leakage channel in Drosophila central neurons

Wright, N. J., Zhong, Y. (January 1998) Serotonin-sensitive leakage channel in Drosophila central neurons. J Neurobiol, 34 (1). pp. 83-95. ISSN 0022-3034 (Print)



This article reports the analysis of a novel serotonin (5-HT)-sensitive leak channel. The 5-HT responses were recorded in acutely dissociated Drosophila adult and larval central nervous system (CNS) neurons by the patch-clamp method, in an attempt to establish a model preparation suitable for the genetic study of signal transduction underlying central neurotransmission. Focal perfusion or iontophoresis of 5-HT onto some patched neurons induced either an apparent inward or outward current. This apparent outward current is able to cause a strong hyperpolarization of the neuron. This article focuses on the predominant hyperpolarizing response, which is observed in a significant fraction of larger CNS neurons and in different developmental stages. The hyperpolarizing response is in fact mediated by inhibiting an inward leak current, which has a reversal potential around 0 mV. This 5-HT-sensitive leak current appears to be mediated mainly by one type of newly identified leak channel with a similar reversal potential of 0 mV and a conductance of 24 pS. In addition, it was also demonstrated that neurotransmitter-induced responses in both larval and adult Drosophila CNS neurons can be analyzed in this acutely dissociated preparation.

Item Type: Paper
Uncontrolled Keywords: Animals Brain cytology physiology Cells, Cultured Drosophila physiology Ganglia Invertebrate cytology physiology Ion Channels physiology Larva physiology Membrane Potentials physiology Neurons physiology Patch-Clamp Techniques Serotonin physiology
Subjects: organism description > animal > insect > Drosophila
Investigative techniques and equipment > patch-clamp recording
Investigative techniques and equipment > recording devices > patch-clamp recording
organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction
CSHL Authors:
Communities: CSHL labs > Zhong lab
Depositing User: CSHL Librarian
Date: January 1998
Date Deposited: 29 Mar 2012 20:44
Last Modified: 29 Mar 2012 20:44

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