Vakoc, C. R., Mandat, S. A., Olenchock, B. A., Blobel, G. A. (2005) Histone H3 lysine 9 methylation and HP1γ are associated with transcription elongation through mammalian chromatin. Molecular Cell, 19 (3). pp. 381-391. ISSN 10972765 (ISSN)
Abstract
Methylation of histones modulates chromatin structure and function. Whereas methylation of histone H3 on lysines 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9 and 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes within euchromatin. Here, we show that H3K9 di- and trimethylation occur in the transcribed region of active genes in mammalian chromatin. This modification is dynamic, as it increases during activation of transcription and is rapidly removed upon gene repression. Heterochromatin Protein 1γ (HP1γ), a protein containing a chromo-domain that recognizes H3K9 methylation, is also present in the transcribed region of all active genes examined. Both the presence of HP1γ and H3K9 methylation are dependent upon elongation by RNA polymerase II. These findings demonstrate novel roles for H3K9 methylation and HP1γ in transcription activation. Copyright ©2005 by Elsevier Inc.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | heterochromatin protein 1 gamma histone H3 protein RNA polymerase II transcription elongation factor unclassified drug animal cell article chromatin controlled study gene activation gene repression heterochromatin mammal mouse nonhuman protein domain RNA methylation transcription initiation Animals Anion Exchange Protein 1, Erythrocyte Cell Line Cell Line Tumor Chromatin Immunoprecipitation Chromosomal Proteins, Non-Histone Dichlororibofuranosylbenzimidazole DNA-Binding Proteins Erythroid Cells Erythroid-Specific DNA-Binding Factors GATA2 Transcription Factor Globins Hela Cells Histones Humans Interleukin-2 Kinetics Liver Lysine Methylation Mice Phosphorylation Protein Binding Protein Isoforms Proto-Oncogene Proteins c-kit Proto-Oncogene Proteins c-myc T-Lymphocytes Transcription Factors Transcription, Genetic Mammalia |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Vakoc lab |
| Depositing User: | CSHL Librarian |
| Date: | 2005 |
| Date Deposited: | 19 Mar 2012 18:38 |
| Last Modified: | 13 Mar 2013 20:34 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25398 |
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