Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution

Cheng, J., Kapranov, P., Drenkow, J., Dike, S., Brubaker, S., Patel, S., Long, J., Stern, D., Tammana, H., Helt, G., Sementchenko, V., Piccolboni, A., Bekiranov, S., Bailey, D. K., Ganesh, M., Ghosh, S., Bell, I., Gerhard, D. S., Gingeras, T. R. (2005) Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution. Science, 308 (5725). pp. 1149-1154. ISSN 00368075 (ISSN) (Public Dataset)

Abstract

Sites of transcription of polyadenylated and nonpolyadenylated RNAs for 10 human chromosomes were mapped at 5-base pair resolution in eight cell lines. Unannotated, nonpolyadenylated transcripts comprise the major proportion of the transcriptional output of the human genome. Of all transcribed sequences, 19.4, 43.7, and 36.9% were observed to be polyadenylated, nonpolyadenylated, and bimorphic, respectively. Half of all transcribed sequences are found only in the nucleus and for the most part are unannotated. Overall, the transcribed portions of the human genome are predominantly composed of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotated transcripts of unknown function. This organization has important implications for interpreting genotype-phenotype associations, regulation of gene expression, and the definition of a gene.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Cell lines Genotype Human chromosomes Transcription Cells Mapping RNA Chromosomes nucleotide genetics article cell line chromosome 13 chromosome 14 chromosome 19 chromosome 20 chromosome 6 chromosome 7 gene expression regulation gene mapping gene sequence genetic transcription human human chromosome human genome phenotype polyadenylation priority journal X chromosome Y chromosome Cell Line, Tumor Cell Nucleus Chromosomes Human Chromosomes Human, Pair 13 Chromosomes Human, Pair 14 Chromosomes Human, Pair 19 Chromosomes Human, Pair 20 Chromosomes Human, Pair 21 Chromosomes Human, Pair 22 Chromosomes Human, Pair 6 Chromosomes Human, Pair 7 Chromosomes Human, X Chromosomes Human, Y Computational Biology Cytosol DNA Complementary DNA, Intergenic Exons Female Genome Human Humans Introns Male Molecular Sequence Data Nucleic Acid Amplification Techniques Oligonucleotide Array Sequence Analysis Physical Chromosome Mapping RNA Splicing RNA, Messenger Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > translation
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 2005
Date Deposited: 14 Mar 2012 16:31
Last Modified: 01 Mar 2013 19:19
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Dataset ID:
URI: https://repository.cshl.edu/id/eprint/25354

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