Efroni, S., Duttagupta, R., Cheng, J., Dehghani, H., Hoeppner, D. J., Dash, C., Bazett-Jones, D. P., Le Grice, S., McKay, R. D. G., Buetow, K. H., Gingeras, T. R., Misteli, T., Meshorer, E. (2008) Global Transcription in Pluripotent Embryonic Stem Cells. Cell Stem Cell, 2 (5). pp. 437-447. ISSN 19345909 (ISSN) (Public Dataset)
Abstract
The molecular mechanisms underlying pluripotency and lineage specification from embryonic stem cells (ESCs) are largely unclear. Differentiation pathways may be determined by the targeted activation of lineage-specific genes or by selective silencing of genome regions. Here we show that the ESC genome is transcriptionally globally hyperactive and undergoes large-scale silencing as cells differentiate. Normally silent repeat regions are active in ESCs, and tissue-specific genes are sporadically expressed at low levels. Whole-genome tiling arrays demonstrate widespread transcription in coding and noncoding regions in ESCs, whereas the transcriptional landscape becomes more discrete as differentiation proceeds. The transcriptional hyperactivity in ESCs is accompanied by disproportionate expression of chromatin-remodeling genes and the general transcription machinery. We propose that global transcription is a hallmark of pluripotent ESCs, contributing to their plasticity, and that lineage specification is driven by reduction of the transcribed portion of the genome. © 2008 Elsevier Inc. All rights reserved.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | STEMCELL animal cell article cell differentiation chromatin assembly and disassembly embryonic stem cell gene activation gene silencing genetic transcription genome male nonhuman plasticity pluripotent stem cell priority journal Animals Cell Lineage DNA, Intergenic Embryonic Stem Cells Epigenesis, Genetic Gene Expression Profiling Humans Mice Pluripotent Stem Cells Transcription, Genetic Transcriptional Activation |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription therapies > stem cells |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 2008 |
| Date Deposited: | 08 Mar 2012 15:14 |
| Last Modified: | 12 Jul 2013 19:46 |
| PMCID: | PMC2435228 |
| Related URLs: | |
| Dataset ID: | |
| URI: | https://repository.cshl.edu/id/eprint/25326 |
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