Kapranov, P., Cawley, S. E., Drenkow, J., Bekiranov, S., Strausberg, R. L., Fodor, S. P. A., Gingeras, T. R. (2002) Large-scale transcriptional activity in chromosomes 21 and 22. Science, 296 (5569). pp. 916-919. ISSN 00368075 (ISSN)
Abstract
The sequences of the human chromosomes 21 and 22 indicate that there are approximately 770 well-characterized and predicted genes. In this study, empirically derived maps identifying active areas of RNA transcription on these chromosomes have been constructed with the use of cytosolic polyadenylated RNA obtained from 11 human cell lines. Oligonucleotide arrays containing probes spaced on average every 35 base pairs along these chromosomes were used. When compared with the sequence annotations available for these chromosomes, it is noted that as much as an order of magnitude more of the genomic sequence is transcribed than accounted for by the predicted and characterized exons.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cells Chromosomes Genes RNA Annotations Genetic engineering polyadenylated RNA chromosome article chromosome 21 chromosome 22 chromosome analysis DNA microarray DNA probe exon gene mapping human priority journal RNA sequence RNA transcription Cell Line Cell Nucleus Chromosomes, Human, Pair 21 Chromosomes, Human, Pair 22 Computational Biology Contig Mapping Cytosol DiGeorge Syndrome DNA, Complementary Exons Humans Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Physical Chromosome Mapping Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Transcription Genetic Tumor Cells Cultured |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > annotation > map annotation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 2002 |
| Date Deposited: | 13 Mar 2012 14:02 |
| Last Modified: | 15 Jul 2013 15:48 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25286 |
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