Kozal, M. J., Shah, N., Shen, N., Yang, R., Fucini, R., Merigan, T. C., Richman, D. D., Morris, D., Hubbell, E., Chee, M., Gingeras, T. R. (1996) Extensive polymorphisms observed in HIV-1 clade B protease gene using high-density oligonucleotide arrays. Nature Medicine, 2 (7). pp. 753-759. ISSN 10788956 (ISSN)
Abstract
Naturally occurring mutations in HIV-1-infected patients have important implications for therapy and the outcome of clinical studies. However, little is known about the prevalence of mutations that confer resistance to HIV-1 protease inhibitors in isolates derived from patients naive for such inhibitors. In the first clinical application of high-density oligonucleotide array sequencing, the sequences of 167 viral isolates from 102 patients have been determined. The DNA sequence of USA HIV-1 clade B proteases was found to be extremely variable and 47.5% of the 99 amino acid positions varied. This level of amino acid diversity is greater than that previously known for all worldwide HIV-1 clades combined (40%). Many of the amino acid changes that are known to contribute to drug resistance occurred as natural polymorphisms in isolates from patients who had never received protease inhibitors.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | proteinase proteinase inhibitor article dna polymorphism drug resistance human human immunodeficiency virus 1 human immunodeficiency virus infection major clinical study priority journal protein polymorphism Amino Acid Sequence Base Sequence Drug Resistance Microbial HIV Infections HIV Protease HIV Protease Inhibitors HIV-1 Humans Molecular Sequence Data Oligonucleotides Polymorphism Genetic Human immunodeficiency virus |
| Subjects: | diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing therapies bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 1996 |
| Date Deposited: | 13 Mar 2012 15:58 |
| Last Modified: | 28 Mar 2014 20:03 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25262 |
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