Detection of mutations associated with zidovudine resistance in human immunodeficiency virus by use of the polymerase chain reaction

Richman, D. D., Guatelli, J. C., Grimes, J., Tsiatis, A., Gingeras, T. R. (1991) Detection of mutations associated with zidovudine resistance in human immunodeficiency virus by use of the polymerase chain reaction. Journal of Infectious Diseases, 164 (6). pp. 1075-1081. ISSN 00221899 (ISSN)

DOI: 10.1093/infdis/164.6.1075


A sensitive and specific polymerase chain reaction (PCR)-based assay was developed for four mutations in the reverse transcriptase gene of human immunodeficiency virus type 1 that have been associated with zidovudine resistance. These mutations were correlated in 366 specimens with zidovudine chemotherapy and resistance. Mutations at these four codons were detected only after zidovudine therapy. The usual sequence of appearance of mutations was codons 215, 70, 67, and 219, although individual variations occurred. The degree of resistance was proportional to the number of mutations present, although variable susceptibilities with identical patterns of mutations suggested the likelihood that additional mutations contribute to resistance. The existence of both phenotypic and genotypic mixtures was documented as was the occasional selection of subpopulations with passage of virus in vitro. The many complexities of zidovudine resistance render the assay of limited use for application to individual patients; however, it could prove useful for correlating disease or therapy with the emergence of resistance.

Item Type: Paper
Additional Information: PubMed ID: 1720152
Uncontrolled Keywords: zidovudine article drug resistance human human cell human immunodeficiency virus infection mutation nonhuman polymerase chain reaction priority journal Acquired Immunodeficiency Syndrome Base Sequence Codon DNA, Viral Drug Resistance, Microbial Genotype HIV Infections HIV-1 HIV-1 Reverse Transcriptase Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Phenotype Predictive Value of Tests Probability Regression Analysis RNA, Viral RNA-Directed DNA Polymerase
Subjects: diseases & disorders > viral diseases > HIV
Investigative techniques and equipment > cloning > PCR
Investigative techniques and equipment > assays > cloning > PCR
diseases & disorders > cancer > drugs and therapies
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1991
Date Deposited: 14 Mar 2012 13:35
Last Modified: 28 Mar 2014 20:43
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