Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris

Tschopp, J. F., Brust, P. F., Cregg, J. M., Stillman, C. A., Gingeras, T. R. (1987) Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Research, 15 (9). pp. 3859-3876. ISSN 03051048 (ISSN)

DOI: 10.1093/nar/15.9.3859


Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase (AOX) and dihydroxy-acetone synthase (DAS) genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for the expression of the Escherichia coli lacZ gene. These vectors were transformed into P. pastoris host cells and employed in experiments to measure the control mechanisms employed by each promoter in the production of β-galactosidase fusion products. Results in P. pastoris suggest that the processes used to regulate the expression of these gene fusions involve both repression/dere-pression and induction mechanisms. Expression of the AOX-lacZ and DAS-lacZ fusions was examined in Saccharomyces cerevisiae as well. Interestingly, β-galactosidase was expressed in a regulated manner in the heterologous host. © 1987 IRL Press Limited, Oxford, England.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: alcohol dehydrogenase alcohol oxidase bacterial protein beta galactosidase formaldehyde transketolase fungal protein galactosidase hybrid protein methanol recombinant protein transferase article biosynthesis comparative study drug effect Escherichia coli fungal gene gene expression regulation genetics Pichia promoter region Saccharomyces Saccharomyces cerevisiae Alcohol Oxidoreductases Bacterial Proteins beta-Galactosidase Fungal Proteins Galactosidases Genes, Fungal Promoter Regions (Genetics) Recombinant Fusion Proteins Recombinant Proteins Saccharomycetales Transferases
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 1987
Date Deposited: 14 Mar 2012 14:12
Last Modified: 23 Jan 2015 14:34
PMCID: PMC340787
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