Gingeras, T. R., Kwoh, D. Y., Davis, G. R. (1987) Hybridization properties of immobilized nucleic acids. Nucleic Acids Research, 15 (13). pp. 5373-5390. ISSN 03051048 (ISSN)
Abstract
The 5′-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency. Results of these experiments reveal that the hybridization efficiencies of support-bound oligonucleotides were 75-80% and 40-50% for single-stranded oligonucleotide targets and long double-stranded targets, respectively. These hybridization efficiencies are dependent upon prehybridizing the support-bound oligonucleotides with dextran sulfate. In addition, comparisons of the relative hybridization efficiencies of the support-bound oligonucleotide and nitrocellulose-based systems have been made which indicate a retention of 13-28% of target sequences on the filters and a detection efficiency of 8-20%. © 1987 IRL Press Limited.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | acrylic acid resin DNA oligodeoxyribonucleotide sephacryl s 500 article kinetics nucleic acid hybridization plasmid Acrylic Resins Oligodeoxyribonucleotides Plasmids |
| Subjects: | Investigative techniques and equipment > comparative genomic hybridization Investigative techniques and equipment > assays > comparative genomic hybridization Investigative techniques and equipment |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 1987 |
| Date Deposited: | 14 Mar 2012 14:09 |
| Last Modified: | 31 Mar 2014 13:49 |
| PMCID: | PMC305967 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25239 |
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