Theriault, G., Roy, P. H., Howard, K. A., Benner, J. S., Brooks, J. E., Waters, A. F., Gingeras, T. R. (1985) Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products. Nucleic Acids Research, 13 (23). pp. 8441-8461. ISSN 03051048 (ISSN)
Abstract
Bal31 delation experiments on clones of the PaeR7 restriction-modification system from Pseudomonas aeruginosa demonstrate that it is arranged as an operon, with the methylase gene preceding the endonuclease gene. The DNA sequence of this operon agrees with in vitro transcription-translation assays which predict proteins of 532 amino acids, Mr = 59,260 daltons, and 246 amino acids, Mr = 27,280 daltons, coincident with the methylase and endonuclease genes, respectively. These predicted values coincide with the measured molecular weights of the purified, denatured PaeR7 endonuclease and methylase proteins. The first twenty amino acids from the amino-terminus of the purified endonuclease exactly match those predicted from the DNA sequence. Finally, potential regulatory mechanisms for the expression of phage restriction are described based on the properties of several PaeR7 subclones. © 1985 IRL Press Limited.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | DNA modification methylase PaeR7 endodeoxyribonuclease XhoI methyltransferase restriction endonuclease amino acid sequence article bacterial gene cell free system Escherichia coli genetic transcription genetics isolation and purification molecular cloning nucleotide sequence plasmid Pseudomonas aeruginosa RNA translation structural gene Base Sequence Cell-Free System Cloning, Molecular DNA Restriction Enzymes Genes, Bacterial Genes, Structural Methyltransferases Plasmids Transcription, Genetic Translation, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > annotation > sequence annotation organism description > bacteria bioinformatics > computational biology |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 1985 |
| Date Deposited: | 14 Mar 2012 13:58 |
| Last Modified: | 31 Mar 2014 14:10 |
| PMCID: | PMC322144 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25236 |
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