Brooks, J. E., Blumenthal, R. M., Gingeras, T. R. (1983) The isolation and characterization of the Esherichia Coli DNA adenine methylase (dam) gene. Nucleic Acids Research, 11 (3). pp. 837-851. ISSN 03051048 (ISSN)
Abstract
The E coli dam (DNA adenine methylase) enxyne is known to nethylate the sequence GATC. A general nethod for cloning sequence-speoific DNA methylase genet was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBK322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dju gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to oode for a protein the sixe of dam. Amino acid conposition derived froa this sequence corresponds closely to the amino acid composition of the purified dam protein. Enxynatic and DNA:DNA hybridixation methods were used to investigate the possible presence of dasi genes in a variety of prokaryotlc organisms. © 1983 IRL Press Limited.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | bacterial DNA DNA Cytosine 5 Methyltransferase DNA cytosine 5 methyltransferase methyltransferase restriction endonuclease amino acid sequence article bacterial gene enzymology Escherichia coli genetics isolation and purification molecular cloning nucleic acid hybridization nucleotide sequence plasmid structural gene Base Sequence Cloning, Molecular DNA Cytosine 5 Methyltransferase DNA Restriction Enzymes DNA, Bacterial Genes Bacterial Genes Structural Methyltransferases Plasmids |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 1983 |
| Date Deposited: | 14 Mar 2012 14:55 |
| Last Modified: | 31 Mar 2014 14:15 |
| PMCID: | PMC325756 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25232 |
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