Cellular factors required for papillomavirus DNA replication

Melendy, T., Sedman, J., Stenlund, A. (December 1995) Cellular factors required for papillomavirus DNA replication. J Virol, 69 (12). pp. 7857-67. ISSN 0022-538X (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/7494298


In vitro replication of papillomavirus DNA has been carried out with a combination of purified proteins and partially purified extracts made from human cells. DNA synthesis requires the viral E1 protein and the papillomavirus origin of replication. The E2 protein stimulates DNA synthesis in a binding site-independent manner. Papillomavirus DNA replication is also dependent on the cellular factors replication protein A, replication factor C, and proliferating-cell nuclear antigen as well as a phosphocellulose column fraction (IIA). Fraction IIA contains DNA polymerase alpha-primase and DNA polymerase delta. Both of these polymerases are essential for papillomavirus DNA replication in vitro. However, unlike the case with T-antigen-dependent replication from the simian virus 40 origin, purified DNA polymerase alpha-primase and delta cannot efficiently replace fraction IIA in the replication reaction. Hence, additional cellular factors seem to be required for papillomavirus DNA replication. Interestingly, replication factor C and proliferating-cell nuclear antigen are more stringently required for DNA synthesis in the papillomavirus system than in the simian virus 40 in vitro system. These distinctions indicate that there must be mechanistic differences between the DNA replication systems of papillomavirus and simian virus 40.

Item Type: Paper
Uncontrolled Keywords: Cell Free System Comparative Study DNA Polymerase III DNA Primase DNA Replication DNA Viral biosynthesis DNA Binding Proteins metabolism DNA-Directed DNA Polymerase metabolism Homeodomain Proteins Humans Kinetics Oncogene Proteins Viral metabolism Papillomavirus genetics metabolism Papillomavirus Bovine genetics metabolism Plasmids Proliferating Cell Nuclear Antigen metabolism Proto Oncogene Proteins c-bc-l2 RNA Nucleotidyltransferases metabolism Replication Factor A Replication Factor C Repressor Proteins Saccharomyces cerevisiae Proteins Simian virus 40 genetics Species Specificity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: December 1995
Date Deposited: 15 Mar 2012 15:19
Last Modified: 14 Feb 2017 20:32
PMCID: PMC189730
Related URLs:
URI: https://repository.cshl.edu/id/eprint/25198

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