The DNA-Binding Domain of Simian Virus-40 Tumor-Antigen Has Multiple Functions

Wun-Kim, K., Upson, R., Young, W., Melendy, T., Stillman, B., Simmons, D. T. (December 1993) The DNA-Binding Domain of Simian Virus-40 Tumor-Antigen Has Multiple Functions. Journal of Virology, 67 (12). pp. 7608-7611. ISSN 0022-538X

DOI: 10.1021/bi982370u


The DNA-binding domain of simian virus 40 tumor antigen has been previously shown to participate in a number of different activities. Besides being involved in binding to sequences at the viral replication origin, this domain appears to be required for nonspecific DNA binding, for structurally distorting origin DNA (melting and untwisting), and possibly for oligomerization of the protein into hexamers and double hexamers. We now provide evidence that it also takes part in unwinding origin DNA sequences, contributes a function specifically related to in vivo DNA replication, and perhaps supports the assembly of the virus or release of the virus from the cell. This 100-amino-acid domain appears to be an excellent model system for studying how a small region of a protein could have a number of distinct activities.

Item Type: Paper
Uncontrolled Keywords: LARGE T-ANTIGEN large T antigen REPLICATION INVITRO replication in vitro HELICASE ACTIVITY helicase activity MONKEY CELLS monkey cells SV40 ORIGIN origin IDENTIFICATION INITIATION initiation HEXAMERS hexamers REGION region
Subjects: diseases & disorders > cancer
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: December 1993
Date Deposited: 17 Feb 2012 19:55
Last Modified: 20 Jun 2017 19:56
PMCID: PMC238227
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