The DNA replication fork in eukaryotic cells

Waga, S., Stillman, B. (1998) The DNA replication fork in eukaryotic cells. Annual Review of Biochemistry, 67. pp. 721-51. ISSN 0066-4154

DOI: 10.1146/annurev.biochem.67.1.721


Replication of the two template strands at eukaryotic cell DNA replication forks is a highly coordinated process that ensures accurate and efficient genome duplication. Biochemical studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA replication, and yeast genetic studies have uncovered the fundamental mechanisms of replication fork progression. At least two different DNA polymerases, a single-stranded DNA-binding protein, a clamp-loading complex, and a polymerase clamp combine to replicate DNA. Okazaki fragment synthesis involves a DNA polymerase-switching mechanism, and maturation occurs by the recruitment of specific nucleases, a helicase, and a ligase. The process of DNA replication is also coupled to cell-cycle progression and to DNA repair to maintain genome integrity.

Item Type: Paper
Uncontrolled Keywords: Cell Cycle DNA Replication Eukaryotic Cells Nuclear Proteins metabolism Nucleic Acid Conformation
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > DNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 1998
Date Deposited: 17 Feb 2012 20:50
Last Modified: 20 Jun 2017 19:31
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