Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence

Tamanoi, F., Stillman, B. W. (November 1983) Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence. Proceedings of the National Academy of Sciences of the United States of America, 80 (21). pp. 6446-50. ISSN 0027-8424

[thumbnail of Stillman_PNAS_1983.pdf]
Stillman_PNAS_1983.pdf - Published Version

Download (1MB) | Preview
DOI: 10.1073/pnas.80.21.6446


Initiation of adenovirus DNA replication in vitro occurs on a linearized plasmid DNA containing 3,327 base pairs of the adenovirus terminal sequence. Various deletions have been constructed in the plasmid DNA and their template activities examined. Deletions from an internal restriction enzyme cleavage site that retain only 20 base pairs or more of the adenovirus terminal sequence support initiation and limited chain elongation, whereas deletions that leave 14 base pairs or less of the terminal sequence do not. On the other hand, all deletions extending from the very terminus of the adenovirus DNA destroy the template activity. The terminal 20 base pairs of adenovirus DNA contain a sequence A-T-A-A-T-A-T-A-C-C, which is perfectly conserved in the DNAs from different serotypes of human adenovirus. Base changes within the conserved sequence greatly reduce the template activity. These results suggest that the terminal 20 base pairs constitute a functional origin for the initiation of adenovirus DNA replication in vitro.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Adenoviruses, Human genetics Base Sequence Chromosome Deletion DNA Replication Plasmids Templates Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: November 1983
Date Deposited: 28 Feb 2012 19:26
Last Modified: 30 Sep 2019 19:47
PMCID: PMC390130
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving