Independent Mutations in Adenovirus Type 2 Ts-111 Cause Degradation of Cellular DNA and Defective Viral DNA Replication

Stillman, B. W., White, E., Grodzicker, T. (1984) Independent Mutations in Adenovirus Type 2 Ts-111 Cause Degradation of Cellular DNA and Defective Viral DNA Replication. Journal of Virology, 50 (2). pp. 598-605.



An adenovirus type 2 mutant, Ad2ts111, has previously been shown to be temperature-sensitive for viral DNA replication in vivo and also to induce degradation of cellular DNA. Soluble nuclear extracts prepared from Ad2ts111-infected human cervical carcinoma HeLa cells grown at either the permissive ( C) or the nonpermissive ( C) temperature are thermolabile for elongation but not for initiation of DNA replication in vitro. Adenovirus single-stranded-DNA-binding protein purified from wild-type-infected cells can complement these extracts at the restrictive temperature in vitro. The DNA-binding protein synthesized in Ad2ts111-infected cells is stable at the nonpermissive temperature and is phosphorylated, as is the wild-type protein. The mutant DNA-binding protein synthesized in Ad5ts125-infected cells is unstable. Ad2ts111 and Ad5ts125 do not complement each other for virus growth in vivo. Evidently, Ad2ts111 contains a mutation the DNA-binding protein that affects viral DNA synthesis. Unlike viral DNA synthesis, the induction of cellular DNA degradation in early region 1 on the virus genome. Thus, the 2 phenotypes displayed in Ad2ts111-infected cells, namely, the temperature-sensitive replication of viral DNA and the degradation of cell DNA, are the result of 2 separate mutations.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
CSHL labs > Grodzicker Lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 1984
Date Deposited: 29 Feb 2012 14:23
Last Modified: 19 Aug 2020 17:19
PMCID: PMC255680
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