The origin recognition complex in silencing, cell cycle progression, and DNA replication

Loo, S., Fox, C. A., Rine, J., Kobayashi, R., Stillman, B., Bell, S. (June 1995) The origin recognition complex in silencing, cell cycle progression, and DNA replication. Molecular and Cellular Biology, 6 (6). pp. 741-56. ISSN 0270-7306

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This report describes the isolation of ORC5, the gene encoding the fifth largest subunit of the origin recognition complex, and the properties of mutants with a defective allele of ORC5. The orc5-1 mutation caused temperature-sensitive growth and, at the restrictive temperature, caused cell cycle arrest. At the permissive temperature, the orc5-1 mutation caused an elevated plasmid loss rate that could be suppressed by additional tandem origins of DNA replication. The sequence of ORC5 revealed a potential ATP binding site, making Orc5p a candidate for a subunit that mediates the ATP-dependent binding of ORC to origins. Genetic interactions among orc2-1 and orc5-1 and other cell cycle genes provided further evidence for a role for the origin recognition complex (ORC) in DNA replication. The silencing defect caused by orc5-1 strengthened previous connections between ORC and silencing, and combined with the phenotypes caused by orc2 mutations, suggested that the complex itself functions in both processes.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cell Cycle genetics Cell Cycle Proteins metabolism Cloning Molecular Crosses, Genetic DNA Replication DNA, Fungal metabolism DNA-Binding Proteins chemistry genetics metabolism physiology Genes Fungal Molecular Sequence Data Mutation Origin Recognition Complex Phenotype Plasmids biosynthesis Polymerase Chain Reaction Repressor Proteins chemistry genetics metabolism/physiology Saccharomyces cerevisiae cytology genetics growth & development metabolism Saccharomyces cerevisiae Proteins Temperature Time Factors Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: June 1995
Date Deposited: 06 Mar 2012 15:15
Last Modified: 20 Jun 2017 19:46
PMCID: PMC301233
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