Loo, S., Fox, C. A., Rine, J., Kobayashi, R., Stillman, B., Bell, S. (June 1995) The origin recognition complex in silencing, cell cycle progression, and DNA replication. Molecular and Cellular Biology, 6 (6). pp. 741-56. ISSN 0270-7306
Preview |
PDF (Paper)
The Origin Recognition Complex in Silencing,.pdf - Published Version Download (3MB) | Preview |
Abstract
This report describes the isolation of ORC5, the gene encoding the fifth largest subunit of the origin recognition complex, and the properties of mutants with a defective allele of ORC5. The orc5-1 mutation caused temperature-sensitive growth and, at the restrictive temperature, caused cell cycle arrest. At the permissive temperature, the orc5-1 mutation caused an elevated plasmid loss rate that could be suppressed by additional tandem origins of DNA replication. The sequence of ORC5 revealed a potential ATP binding site, making Orc5p a candidate for a subunit that mediates the ATP-dependent binding of ORC to origins. Genetic interactions among orc2-1 and orc5-1 and other cell cycle genes provided further evidence for a role for the origin recognition complex (ORC) in DNA replication. The silencing defect caused by orc5-1 strengthened previous connections between ORC and silencing, and combined with the phenotypes caused by orc2 mutations, suggested that the complex itself functions in both processes.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cell Cycle genetics Cell Cycle Proteins metabolism Cloning Molecular Crosses, Genetic DNA Replication DNA, Fungal metabolism DNA-Binding Proteins chemistry genetics metabolism physiology Genes Fungal Molecular Sequence Data Mutation Origin Recognition Complex Phenotype Plasmids biosynthesis Polymerase Chain Reaction Repressor Proteins chemistry genetics metabolism/physiology Saccharomyces cerevisiae cytology genetics growth & development metabolism Saccharomyces cerevisiae Proteins Temperature Time Factors Transcription Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | June 1995 |
| Date Deposited: | 06 Mar 2012 15:15 |
| Last Modified: | 20 Jun 2017 19:46 |
| PMCID: | PMC301233 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25007 |
Actions (login required)
 |
Administrator's edit/view item |