Orc1 Controls Centriole and Centrosome Copy Number in Human Cells

Hemerly, A. S., Prasanth, S. G., Siddiqui, K., Stillman, B. (February 2009) Orc1 Controls Centriole and Centrosome Copy Number in Human Cells. Science, 323 (5915). pp. 789-793.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19197067
DOI: 10.1126/science.1166745


Centrosomes, each containing a pair of centrioles, organize microtubules in animal cells, particularly during mitosis. DNA and centrosomes are normally duplicated once before cell division to maintain optimal genome integrity. We report a new role for the Orc1 protein, a subunit of the origin recognition complex (ORC) that is a key component of the DNA replication licensing machinery, in controlling centriole and centrosome copy number in human cells, independent of its role in DNA replication. Cyclin A promotes Orc1 localization to centrosomes where Orc1 prevents Cyclin E-dependent reduplication of both centrioles and centrosomes in a single cell division cycle. The data suggest that Orc1 is a regulator of centriole and centrosome reduplication as well as the initiation of DNA replication.

Item Type: Paper
Uncontrolled Keywords: ORIGIN RECOGNITION COMPLEX origin recognition complex CYCLIN-E Orc1 Cyclin-E DNA-REPLICATION DNA replication DUPLICATION duplication AMPLIFICATION amplification OVERDUPLICATION overduplication LOCALIZATION localization ACCUMULATION accumulation CDK2-CYCLIN CDK2 cyclin LICENSES licenses
Subjects: organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 6 February 2009
Date Deposited: 06 Mar 2012 15:59
Last Modified: 03 Oct 2019 13:32
PMCID: PMC2653626
Related URLs:
URI: https://repository.cshl.edu/id/eprint/24986

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