Hemerly, A. S., Prasanth, S. G., Siddiqui, K., Stillman, B. (February 2009) Orc1 Controls Centriole and Centrosome Copy Number in Human Cells. Science, 323 (5915). pp. 789-793.
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Abstract
Centrosomes, each containing a pair of centrioles, organize microtubules in animal cells, particularly during mitosis. DNA and centrosomes are normally duplicated once before cell division to maintain optimal genome integrity. We report a new role for the Orc1 protein, a subunit of the origin recognition complex (ORC) that is a key component of the DNA replication licensing machinery, in controlling centriole and centrosome copy number in human cells, independent of its role in DNA replication. Cyclin A promotes Orc1 localization to centrosomes where Orc1 prevents Cyclin E-dependent reduplication of both centrioles and centrosomes in a single cell division cycle. The data suggest that Orc1 is a regulator of centriole and centrosome reduplication as well as the initiation of DNA replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | ORIGIN RECOGNITION COMPLEX origin recognition complex CYCLIN-E Orc1 Cyclin-E DNA-REPLICATION DNA replication DUPLICATION duplication AMPLIFICATION amplification OVERDUPLICATION overduplication LOCALIZATION localization ACCUMULATION accumulation CDK2-CYCLIN CDK2 cyclin LICENSES licenses |
| Subjects: | organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 6 February 2009 |
| Date Deposited: | 06 Mar 2012 15:59 |
| Last Modified: | 03 Oct 2019 13:32 |
| PMCID: | PMC2653626 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24986 |
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