Cellular factors required for multiple stages of SV40 DNA replication in vitro

Fairman, M. P., Stillman, B. (April 1988) Cellular factors required for multiple stages of SV40 DNA replication in vitro. Embo Journal, 7 (4). pp. 1211-8. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/2841119


Plasmids containing the SV40 origin replicate in the presence of SV40 T antigen and a cell free extract derived from human 293 cells. Upon fractionation of this extract, two essential replication factors have been identified. One of these is a multi-subunit DNA binding protein containing polypeptides of 70,000, 34,000 and 11,000 daltons which may function as a eukaryotic single strand DNA binding protein (SSB). The other partially purified fraction is required with T antigen for the first stage of DNA replication, the formation of a pre-synthesis complex at the replication origin. These results, and others, define multiple stages of SV40 DNA replication in vitro which are analogous to multiple stages of Escherichia coli and phage lambda replication, and may reflect similar events in the replication of cellular chromosomes.

Item Type: Paper
Uncontrolled Keywords: Antigens Polyomavirus Transforming genetics Cell Line Cell Transformation Viral DNA Replication Genes, Viral Humans Kinetics Plasmids Simian virus 40 genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: April 1988
Date Deposited: 06 Mar 2012 16:51
Last Modified: 20 Jun 2017 20:30
PMCID: PMC454458
URI: https://repository.cshl.edu/id/eprint/24977

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