Diffley, J. F., Stillman, B. (February 1992) DNA binding properties of an HMG1-related protein from yeast mitochondria. Journal of Biological Chemistry, 267 (5). pp. 3368-74. ISSN 0021-9258
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Abstract
The DNA binding properties of ABF2, an abundant protein found in the mitochondria of the yeast Saccharomyces cerevisiae have been examined in detail. ABF2 is closely related to the vertebrate high mobility group protein HMG1 and like HMG1, ABF2 will introduce negative supercoils into a relaxed, double-stranded circular DNA molecule in cooperation with a DNA topoisomerase. Additionally, ABF2 binds approximately 5-10 times more tightly to negatively supercoiled DNA than to relaxed circular or linear DNA. Although ABF2 binds to most random double-stranded sequences with roughly equal affinity, its binding within certain key regulatory regions is qualitatively quite different. First, ABF2 binding induces a distinct pattern of DNA bending within the chromosomal origin of DNA replication, ARS1. Second, ABF2 binding to all nuclear replication origins tested, in addition to a critical mitochondrial promoter and replication origin, is clearly nonrandom as visualized by DNase1 footprinting. Analysis of the sequences found within these regions as well as competition experiments with synthetic DNA molecules suggest that site-specific DNA binding may be accomplished by the phased distribution of short stretches of poly(dA), which exclude ABF2 binding. These patterns of ABF2 DNA binding suggest a role for the protein in genome organization and site-specific regulation of transcription or DNA replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence DNA Superhelical metabolism DNA Binding Proteins genetics isolation & purification metabolism Fungal Proteins genetics isolation & purification metabolism High Mobility Group Proteins metabolism Kinetics Mitochondria metabolism Molecular Sequence Data Plasmids Protein Binding Restriction Mapping Saccharomyces cerevisiae metabolism Saccharomyces cerevisiae Proteins Transcription Factors |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 15 February 1992 |
| Date Deposited: | 07 Mar 2012 16:28 |
| Last Modified: | 20 Jun 2017 20:03 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24955 |
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