Similarity between the transcriptional silencer binding proteins ABF1 and RAP1

Diffley, J. F., Stillman, B. (November 1989) Similarity between the transcriptional silencer binding proteins ABF1 and RAP1. Science, 246 (4933). pp. 1034-8. ISSN 0036-8075



The yeast ARS binding factor 1 (ABF1)--where ARS is an autonomously replicating sequence--and repressor/activator protein 1 (RAP1) have been implicated in DNA replication, transcriptional activation, and transcriptional silencing. The ABF1 gene was cloned and sequenced and shown to be essential for viability. The predicted amino acid sequence contains a novel sequence motif related to the zinc finger, and the ABF1 protein requires zinc and unmodified cysteine residues for sequence-specific DNA binding. Interestingly, ABF1 is extensively related to its counterpart, RAP1, and both proteins share a region of similarity with SAN1, a suppressor of certain SIR4 mutations, suggesting that this region may be involved in mediating SIR function at the silent mating type loci.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Comparative Study DNA-Binding Proteins genetics Fungal Proteins genetics Genes, Structural Fungal Metalloproteins genetics Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Transcription Factors Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > annotation > sequence annotation
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 24 November 1989
Date Deposited: 07 Mar 2012 16:16
Last Modified: 20 Jun 2017 20:17
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving